TABLE L—Record of fishing at Grand Lake Stream, Maine, October and November, 1877—Continued.

Treatment as usual. Treatment as usual, except a much heavier milting. Treatment as usual, except great pains to handle in small lots and secure contact between milt and spawn.

The eggs were weighed in the spawn-pans just before applying water, and therefore included not only the liquid that came with them from the mother-fish, but also what milt had been used to impregnate them. To obtain data to estimate the ratio of impregnation, a sample, numbering from 50 to 200, was taken from each lot, and each egg of the sample carefully examined. I am convinced, however, that the number examined was too small for the purpose. The percentages stated must therefore not be regarded as very accurate, but as only indicating the ratio in a general way.

Estimated.

TABLE II.-Record of spawning operations, Grand Lake Stream, 1877—Continued.

*The eggs were weighed in the spawn-pans just before applying water, and therefore included not only the liquid that came with them from the mother-fish, but also what milt had been used to impregnate them. To obtain data to estimate the ratio of impregnation, a sample, numbering from 50 to 200, was taken from each lot, and each egg of the sample carefully examined. I am convinced, however, that the number examined was too small for the purpose. The percentages stated must therefore not be regarded as very accurate, but as only indicating the ratio in a general way.

I am inclined to think that the eggs taken this season (and other seasons as well) were overestimated 5 per cent., perhaps more.